Part:BBa_K874060:Design

Methylation Site (M.ScaI & dual E2C targeted)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 35
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Contains no Forbidden & Undesirable restriction site specified by the RFC-10 protocol.
Also contains no sites recognised by native E.Coli restriction/methylation systems.

Source

Synthesized by IDT.